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KMID : 0381120080300050469
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Genes and Genomics
2008 Volume.30 No. 5 p.469 ~ p.476
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Identification of PABPN1 as an Interactive Protein of hnRNP L via Yeast Two-Hybrid Screening
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Choi Mie-Young
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Abstract
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Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is one of the primary pre-mRNA binding proteins found in human cells. The hnRNP L protein is an abundant nucleocytoplasmic shuttling protein. In an attempt to identify the binding partners of hnRNP L, yeast two-hybrid screening was conducted using a HeLa cDNA library. One of the cDNA clones was determined to harbor a partial human nuclear poly(A)-binding protein (PABPN1) (GenBank accession number NM_004643). For the first time in this study, hnRNP L is shown to interact specifically with the PABPN1 in the yeast two-hybrid system. The in vitro analysis of the interaction occurring between hnRNP L and PABPN1 was also carried out. The results imply that, even though hnRNP L is not able to directly interact with PABPN1 in vitro, protein-protein association between hnRNP L and PABPN1 can be formed by protein-RNA-protein interactions.
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KEYWORD
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heterogeneous nuclear ribonucleoproteins (hnRNPs), RNP-concensus RNA-binding domain, yeast two-hybrid screening system, nuclear poly(A)-binding protein (PABPN1)
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